Description
The Human Carboxypeptidase A2 / CPA2 ELISA Pair Set is for the quantitative determination of Human Carboxypeptidase A2 / CPA2. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Carboxypeptidase A2 / CPA2 coated on a 96-well plate. Standards and samples are added to the wells, and any Carboxypeptidase A2 / CPA2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbi anti-Carboxypeptidase A2 / CPA2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Carboxypeptidase A2 / CPA2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm